The elisa technical route ELISA is based on the immobilization of antigens or antibodies and the enzymatic labeling of antigens or antibodies. The antigen or antibody bound to the surface of the solid phase carrier of the ELISA kit retains its immunological activity, and the enzyme-labeled antigen or antibody retains both its immunological activity and the activity of the enzyme. At the time of the assay, the ELISA kit test sample (the antibody or antigen assayed therein) reacts with the antigen or antibody on the surface of the solid phase carrier. The antigen-antibody complex formed on the solid support is separated from other substances in the liquid by washing. Further, an enzyme-labeled antigen or antibody is added, and is also bound to the solid phase carrier by a reaction. At this time, the amount of enzyme on the solid phase is proportional to the amount of the substance to be tested in the specimen. After the substrate of the enzyme reaction is added, the substrate is catalyzed by the enzyme to become a colored product, and the amount of the product is directly related to the amount of the test substance in the sample, so that qualitative or quantitative analysis can be performed according to the depth of the color. Due to the high catalytic efficiency of the enzyme, the result of the immune reaction is indirectly amplified, and the measurement method achieves high sensitivity. ELISA can be used to determine antigens and can also be used to determine antibodies.
However, there are many factors affecting the results of the Elisa test, so it is necessary to strengthen the quality assurance of each link to fully utilize the advantages of its methodology.
Advantages of ELISA kits 1. Highly efficient, sensitive and specific antibodies;
Second, stable repeatability and reliability;
3. A solid phase carrier with good adsorption performance, low blank value and high transparency at the bottom of the hole;
Fourth, the application of serum, plasma, tissue homogenate, cell culture supernatant, urine and other types of specimens;
Fifth, save experimental funds.
Development of ELISA kits The development of clinical assay technology is mainly due to the development of methodology, and the development of methodology relies on the continuous advancement of reagent production technology and the application of type markers. At present, molecular biology is and will eventually give us a comprehensive and thorough understanding of the entire life sciences. Its impact on the development of immunoassay technology is also direct and effective. The ELISA kit makes us difficult to detect before. The determination of biologically active substances becomes a breeze and greatly increases the sensitivity and specificity of the detection.
This model of a lima bean leaf vividly shows the transverse and longitudinal sections, as well as the upper and lower surfaces, of a typical leaf. The mesophyll includes palisade parenchyma and spongy parenchyma. Both surfaces are shown with stomata and guard cells. Vascular bundle detail is also shown,including xylem, phloem, and veins.
the model produced by the company has been widely used in many educational system units, such as medical and health, medical education, Red Cross, electric power, transportation, fire protection, safety production, public community and so on, and has occupied a gratifying market share, which has been favored and praised by customers.
Plant Model,3D Plant Model,Anatomy 3D Model,Plant Stem Cell Model
Xinxiang Vic Science&Education Co.,Ltd. , https://www.hnlaboratoryequipment.com