Shanghai Haozhuang Instrument Co., Ltd. Haozhuang (LNB) PCR is a large amount of In Vitro synthesis of specific genes using DNA polymerase in vitro or in a test tube. Basically, it uses DNA polymerase for specific chain replication. At present, the commonly used technology can copy a gene to 10 billion to 100 billion times. According to the purpose of DNA amplification and detection standards, the PCR instrument can be divided into four categories: ordinary PCR instrument, gradient PCR instrument, in-situ PCR instrument, and real-time fluorescence quantitative PCR instrument.
How to use the gene amplification PCR instrument:
PCR consists of three basic reaction steps: denaturation-annealing (renaturation)-extension: ①Denaturation of template DNA: After template DNA is heated to 90 ~ 95 ℃ for a certain period of time, template DNA is double-stranded or formed by PCR amplification The double-stranded DNA is dissociated to make it single-stranded, so that it can be combined with the primer to prepare for the next round of reaction; ② Annealing of template DNA and primer (renaturation): After the template DNA is denatured into single-strand by heating, the temperature drops At 55 ~ 60 ℃, the primer is paired with the complementary sequence of the single-stranded DNA of the template DNA; ③ Extension of the primer: DNA template-primer conjugate under the action of DNA polymerase at 70 ~ 75 ℃, using dNTP as the reaction raw material, The target sequence is the template. According to the principles of base pairing and semi-reserved replication, a new semi-reserved replication strand complementary to the template DNA strand is synthesized to repeat the cycle of denaturation-annealing-extension, and more "semi-reserved" can be obtained. Copy chain ", and this new chain can become a template for the next cycle. Each cycle takes 2 to 4 minutes and 2 to 3 hours to amplify the target gene to be amplified by several million times.
The PCR instrument of Shanghai Haozhuang Instrument Co., Ltd. has fast temperature rise and fall, and the temperature is accurate and even. There are many models. Welcome to inquire!
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