Pesticide residue speed test card use method

The pesticide rapid test card is a convenient and highly sensitive analytical tool that uses cholinesterase and a chromogenic reagent to detect the presence of organophosphate and carbamate pesticides in vegetables. This method offers excellent resistance to interference, making it ideal for quick on-site screening without the need for complex equipment or specialized training. The test card is easy to store, transport, and use, making it an efficient solution for field testing. **Instructions:** **Overall Assay Method** 1. Select a representative vegetable sample. Remove surface dirt and cut it into small pieces (about 1 cm²). Take 5 grams of the sample and place it in a sealed container. Add 10 mL of distilled water or buffer solution, shake the container about 50 times, then let it sit for at least 2 minutes. 2. Take a rapid test card and use the white tablet to absorb the extracted liquid. Allow the tablet to react for more than 10 minutes. Then place it in a 37°C incubator for 10 minutes. Ensure the surface of the tablet remains moist throughout the process. 3. Fold the test card in half and hold it for 3 minutes, or place it in a 37°C incubator for the same duration, allowing the red and white tablets to overlap. 4. Always include a blank control card using purified water or buffer solution for each batch of testing. **Surface Measurement Method (Rough Screening)** 1. Wipe the surface of the vegetable to remove any dirt. Apply 2-3 drops of the extraction solution onto the vegetable’s surface, and gently rub it with another vegetable. 2. Take a rapid test card and apply the same drops onto the white tablet. 3. Let the tablet react for over 10 minutes, then place it in a 37°C incubator for 10 minutes. Keep the tablet surface moist during this time. 4. Fold the card in half and hold it for 3 minutes or use the incubator for the same period to bring the red and white tablets together. 5. Include a blank control card using the extraction solution for each batch. **Result Interpretation:** Compare the white tablet with the blank control. If the white tablet remains unchanged or shows a slight blue color, it indicates a negative result. A strong blue color means a positive result, suggesting high pesticide residue. A light blue color indicates a weak positive, meaning low levels of pesticide are present. If the white tablet turns sky blue or matches the blank control, it is considered negative. For samples showing positive results, further analysis using other methods is recommended to identify the specific type and concentration of the pesticide.

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