Pesticide residue speed test card use method

The pesticide rapid test card is a convenient and sensitive tool designed to detect organophosphate and carbamate pesticides in vegetables. It works by utilizing cholinesterase and a chromogenic reagent, which react with pesticide residues, making it an effective method for on-site screening. This test is highly selective, user-friendly, and doesn't require any specialized equipment or extensive training. The cards are easy to store, transport, and use, making them ideal for quick inspections in the field. **Instructions:** **Overall Assay Method** 1. Select a representative vegetable sample, wipe off surface dirt, cut into small pieces (about 1 cm²), and place 5 grams into a sealed container. Add 10 mL of distilled water or buffer solution, shake thoroughly for 50 times, and let it sit for at least 2 minutes. 2. Take a test card and apply the extracted liquid onto the white tablet. Allow it to pre-react for more than 10 minutes. Then place it in a 37°C incubator for 10 minutes. Ensure the tablet remains moist during this process. 3. Fold the test card in half and hold it for 3 minutes, or place it in a 37°C incubator for the same duration to allow the red and white tablets to overlap. 4. Always include a blank control card using purified water or buffer solution for each batch of testing. **Surface Screening Method (Quick Test)** 1. Wipe the surface of the vegetable to remove dirt. Apply 2–3 drops of the eluent onto the vegetable surface and gently rub it against another vegetable. 2. Take a test card and apply the drops from the vegetable onto the white tablet. 3. Let the tablet pre-react for over 10 minutes, then place it in a 37°C incubator for 10 minutes. Keep the tablet moist throughout the process. 4. Fold the card in half and hold it for 3 minutes or incubate for the same time to bring the red and white tablets together. 5. Include a blank control card for the eluent in every test batch. **Result Interpretation:** Compare the white tablet with the blank control. If the tablet remains unchanged or shows a slight blue color, it is considered negative. A light blue color indicates a weak positive result, suggesting low pesticide residue. A strong blue color means a high level of pesticide contamination. If the white tablet turns sky blue or matches the blank control, it is negative. For samples showing positive results, further laboratory analysis should be conducted to identify the specific type and concentration of pesticides present.

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