[Human hepatitis B virus X antigen (HBxAg) elisa technology] is used to determine the content of hepatitis B virus X antigen (HBxAg) in human serum, plasma and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of human hepatitis B virus X antigen (HBxAg) in the specimen. The microplate is coated with the purified antibody to prepare a solid phase antibody, and the hepatitis B virus X antigen (HBxAg) is sequentially added to the microcapsule of the coated monoclonal antibody, and then combined with the HRP-labeled antibody to form an antibody-antigen-enzyme. The standard antibody complex was thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with the hepatitis B virus X antigen (HBxAg) in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader, and the concentration of human hepatitis B virus X antigen (HBxAg) in the sample was calculated from a standard curve. [Human hepatitis B virus X antigen (HBxAg) elisa technology] rat lipoprotein alpha (Lp-α) production elisa kit human unmethylated oligodeoxynucleotide (CpG-ODN) production elisa kit rabbit nerve Growth factor (NGF) production elisa kit human extracellular matrix phosphoglycoprotein (MEPE) production elisa kit chicken interleukin 2 (IL-2) production elisa kit crystal violet neutral red bile salt agar (VRBA) chicken main tissue phase Capacitive complex (MHC/B) production of elisa kit human NOD-like receptor (NLR) production elisa kit [human hepatitis B virus X antigen (HBxAg) elisa technology]
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