R & D Systems offers a complete range of complete ELISA kits. To ensure high quality and repeatability, all kits have undergone extremely strict quality inspections. To detect intracellular and extracellular proteins, the kit uses a variety of detection methods, including colorimetry, fluorescence and chemiluminescence.
Specification: 48 times / 96 times Price: 1500/2500
96 times kit composition:
1 30 times concentrated washing solution 20ml × 1 bottle 7 stop solution 6ml × 1 bottle
2 Enzyme label reagent 6ml × 1 bottle 8 standard (pg / ml) 0.5ml × 1 bottle
3 Enzyme label coating plate 12 wells × 8 strips 9 Standard diluent 1.5ml × 1 bottle
4 Sample diluent 6ml × 1 bottle 10 Instructions 1 copy
5 Developer A solution 6ml × 1 bottle 11 sealing film 2 sheets
6 Developer B liquid 6ml × 1 / bottle 12 sealed bag 1
Composition of 48 kits:
1 20 times concentrated washing solution 20ml × 1 bottle 7 stop solution 3ml × 1 bottle
2 Enzyme label reagent 3ml × 1 bottle 8 standard (pg / ml) 0.5ml × 1 bottle
3 Enzyme label coating plate 12 well × 4 strips 9 Standard dilution 1.5ml × 1 bottle
4 Sample diluent 3ml × 1 bottle 10 Instructions 1 copy
5 Developer A solution 3ml × 1 bottle 11 sealing film 2 sheets
6 Developer B liquid 3ml × 1 / bottle 12 sealed bag 1
Storage conditions: 2-8 degrees
Specimens: including serum, plasma, urine, pleural and ascites fluid, cerebrospinal fluid, cell culture supernatant, tissue homogenate, etc. Provide free testing services.
Test results within one week
Specimen collection method:
1. Serum:
After the blood coagulates naturally at room temperature for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.
2. Plasma:
EDTA, sodium citrate or heparin should be selected as the anticoagulant according to the requirements of the specimen, mixed for 10-20 minutes, and centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.
3. Urine:
Collect with sterile tubes. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again. Pleural and ascites, cerebrospinal fluid refer to this practice.
4. Cell culture supernatant:
When detecting secreted components, collect them with sterile tubes. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully.
5. Cultivation of cells When testing the contents of cells, dilute the cell suspension with PBS (PH7.2-7.4) to a cell concentration of about 1 million / ml. By repeatedly freezing and thawing or adding tissue protein extraction reagents, the cells are destroyed and the intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.
6. Organize the specimen After cutting the specimen, weigh it. Add a certain amount of PBS, PH7.4. Quickly freeze and save with liquid nitrogen for later use. After the specimen melts, it still maintains a temperature of 2-8 ° C. Add a certain amount of PBS (PH7.4), or tissue protein extraction reagent, and homogenize the specimen by hand or homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. After aliquoting, a portion is to be tested, and the rest is frozen for future use.
The company has a complete range of kits, including humans, rats, mice, rabbits, sheep, horses, cattle, guinea pigs, monkeys, fish, plants, food, etc. Nearly 1,000 kinds of kits have been put on the market, and are widely used by researchers Use, favored by customers
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