Rat immunoglobulin G (IgG) Elisa kit

Posted on

Rat immunoglobulin G (IgG) Elisa kit

Rat immunoglobulin G (IgG) Elisa kit instructions
The rat immunoglobulin G (IgG) Elisa kit is for research use only.
Detection range: 96T 0.5μg / ml -12μg / ml
Purpose: Rat immunoglobulin G (IgG) Elisa kit is used to determine the content of immunoglobulin G (IgG) in rat serum, plasma and related liquid samples.
Experimental principle: This kit uses the double antigen sandwich method to determine the level of rat immunoglobulin G (IgG) in the specimen. The microplate is coated with purified antigen to make a solid-phase antigen. Immunoglobulin G (IgG) is added to the monoclonal antibody-coated microwells in turn, and then combined with the HRP-labeled antigen to form an antigen-antibody-enzyme-labeled antigen After thorough washing, the compound is added with TMB to develop color. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the immunoglobulin G (IgG) in the sample. Measure the absorbance (OD value) at 450nm with a microplate reader, pass the standard
The curve calculates the concentration of rat immunoglobulin G (IgG) in the sample.
The composition of rat immunoglobulin G (IgG) Elisa kit:
1 30 times concentrated washing solution 20ml × 1 bottle 7 stop solution 6ml × 1 bottle
2 Enzyme label reagent 6ml × 1 bottle 8 standard (24μg / ml) 0.5ml × 1 bottle
3 Enzyme label coated plate 12 wells × 8 strips 9 standard dilutions 1.5ml × 1 bottle
4 Sample diluent 6ml × 1 bottle 10 instructions 1 copy
5 Developer A solution 6ml × 1 bottle 11 2 sealing film
6 Developer B liquid 6ml × 1 / bottle 12 1 sealed bag
Specimen requirements:
1. The sample containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP).
2. The specimens should be extracted as soon as possible after collection. The extraction should be carried out according to relevant literature. If the test cannot be performed immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided
Steps:
1. Dilution of standard products: This kit provides one original standard product. The user can perform dilution in a small test tube according to the following chart.
12μg / ml No. 5 standard 150μl original standard added 150μl standard dilution
6μg / ml No. 4 standard 150μl No. 5 standard added 150μl standard dilution
3μg / ml No. 3 standard 150μl No. 4 standard added 150μl standard dilution
1.5μg / ml No. 2 standard 150μl No. 3 standard added 150μl standard diluent
0.75μg / ml No. 1 standard 150μl No. 2 standard added 150μl standard dilution
2. Add samples: set up blank wells (the blank control wells do not add samples and enzyme reagents, the rest of the steps are the same), standard wells, sample wells to be tested. Accurately add 50μl of standard on the enzyme-coated plate, add 40μl of sample diluent to the sample well, and then add 10μl of sample to be tested (the final dilution of the sample is 5 times) Add the sample and add the sample to the bottom of the well of the microplate, try not to touch the wall of the well, shake gently to mix.
3. Incubation: seal the plate with the sealing film and incubate at 37 ° C for 30 minutes.
4. Mixing solution: Dilute 30 times concentrated washing liquid with distilled water 30 times and reserve
5. Washing: Carefully peel off the sealing film, discard the liquid, spin dry, fill each well with the washing liquid, let it stand for 30 seconds, then discard, repeat 5 times and pat dry.
6. Add enzyme: add 50μl of enzyme label reagent to each well, except blank well.
7. Incubation: The operation is the same as 3.
8. Washing: The operation is the same as 5.
9. Color development: Add 50μl of developer A to each well, and then add 50μl of developer B, mix gently, and develop for 15 minutes in the dark at 37 ℃.
10. Termination: Add 50μl of stop solution to each well to terminate the reaction (at this time, the blue color turns to yellow).
11. Determination: Measure the absorbance (OD value) of each well in sequence with the blank air conditioner at zero and 450nm wavelength. The measurement should be carried out within 15 minutes after adding the stop solution.
Calculation: Taking the concentration of the standard as the abscissa and the OD value as the ordinate, draw a standard curve on the graph paper, and find the corresponding concentration from the standard curve according to the OD value of the sample; then multiply by the dilution factor; The linear regression equation of the standard curve is calculated by the concentration and OD value of the sample, and the OD value of the sample is substituted into the equation to calculate the sample concentration, and then multiplied by the dilution factor, which is the actual concentration of the sample.
Precautions:
1. The kit should be taken out of the refrigerated environment and equilibrated at room temperature for 15-30 minutes before use. If the enzyme-coated plate is unsealed after opening, the strip should be stored in a sealed bag.
2. Crystals may be precipitated in the concentrated washing liquid, which can be heated and dissolved in a water bath during dilution, and the results will not be affected during washing.
3. The sampler should be used at each step of sample addition, and the accuracy should be regularly checked to avoid test errors. It is best to control the sampling time within 5 minutes. If there are many specimens, it is recommended to use a volley gun to add samples.
4. Please make a standard curve at the same time of each measurement, it is best to make a double hole. If the content of the test substance in the specimen is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with a certain multiple of the sample diluent (n times) before measuring, and finally multiply by the total dilution Multiple (× n × 5).
5. The sealing film is limited to one-time use to avoid cross-contamination.
6. Please keep the substrate away from light.
7. Strictly follow the instructions, and the test results must be determined by the microplate reader.
8. All samples, washing liquids and various wastes should be treated as infectious agents.
9. The components of different batches of this reagent shall not be mixed.
10. If there is any difference with the English manual, the English manual shall prevail.
Storage conditions and validity period:
1. Kit storage: 2-8 ° C.
2. Validity: 6 months

Household Ladder- Steps Ladder - aluminum household stepladder for general use. It`s comfortable, double rivetted treads, heavy duty locking side stays and tubular back legs for strength and rigidity .... featuring anti slip rubber feet and an all aluminum top. Easy to extend and fold 2 steps , ,3 steps ,4 steps ,5 steps ,6 steps ,7 steps ,8 steps ,9 steps .It`s lightweight and portable aluminum design.Conforming to EN131. The max load of Household ladder is 150KGS.

Choose the Household ladder without hesitation as it would be the most convenient assistant for your life .

More details as follows :

Household LadderHousehold Ladder

Household Ladder

Household Ladder

Household Ladder

FAQ:

Q: Are you factory or trading company ?

A: We are factory , exported foreign over decade with rich experiences .

Q: We can change the color of the rubber cover ?

A: yes , the regular is black , you also choose other color .

Q:What`s the MOQ ?

A: 50 PCS.

Q: Can I have a sample ?

A: It`s ok .

Q:What`s your price ?

A: It`s depends on your demands, quantity , we can offer you lowest price .

Q:What`s your delivery time ?

A: Sample order : 3days , bulk order : 23-25days .

Q: Can we use our logo or brand on the package ?

A: yes , OEM is ok .

Q: What`s the payment :

A: (1) T/T: 30% deposit be paid in advance , the 70% of total payment see the copy of B/L.

(2) L/C it`s ok .

  (3) Cash also ok .

 

 

More inquires or questions , plz feel free to contact us !

Welcome to visit our factory !




Household Ladder

Household Ladder,Aluminum Household Ladder,2 Step Household Ladder,Steps Household Ladder

Yongkang Aoyi Industry&Trade Co., Ltd. , https://www.aoyiladder.com