Identification of ELISA results

Identification of ELISA results, accuracy: determination of immunological specificity. The purity of antigens and antibodies was determined by immunodiffusion, cross-electrophoresis and immunoelectrophoresis. For the determination of antigen (or hapten), the results of radioimmunoassay (RIA) are generally compared; for the determination of antibodies, the results of immunofluorescence (IFA) are generally compared. Use the data obtained from other experiments to verify. For example, after the virus infects animals and plants, a series of pathological changes will occur. If there is a linear relationship between these pathological data and the amount of virus infection, the amount of virus measured by ELISA should be consistent with the changes in pathological data. For multiple experiments, compare the results of each ELISA. 2. Accuracy: A series of different concentrations of the same sample are measured to obtain a curve with a wide concentration range. The steep slope and good repeatability of the curve are selected as the measurement range. The determination of the ELISA measurement range is useful for choosing the appropriate concentration for practical use. 3. Spirituality: The sensitivity of ELISA is high, and its sensitivity to the determination of many antigens can reach 1-10ng / ml. It is prudent to draw conclusions about the sensitivity of a certain method of ELISA, because many factors will affect the sensitivity of ELISA, such as antibody titer, enzyme activity, enzyme-labeled antibody quality, reaction time and temperature, carrier adsorption force, substrate Concentration and pH etc. A comparison can only be made if the conditions are strictly consistent.

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