Thrombin-Activated Fibrinolysis Inhibitor (TAFI) Kit Instructions:
Before starting the test, take the kit out of the refrigerator and allow it to reach room temperature for 15–30 minutes. If the enzyme-coated strip is not used immediately after opening, store it in a sealed bag to maintain its integrity.
The concentrated washing solution may crystallize during storage. When preparing the working solution, you can gently heat it in a water bath to dissolve the crystals. This will not affect the test results.
Use a dedicated pipette for each step, and ensure accuracy by calibrating regularly to prevent errors. It’s recommended to complete the sample loading within 5 minutes. For large numbers of samples, using a multichannel pipette is highly advised.
Always prepare a standard curve alongside your samples. It’s best to run each standard in duplicate. If the OD value of your sample exceeds that of the highest standard well, dilute the sample before testing. Remember to multiply the final result by the dilution factor (e.g., ×n×5) when calculating.
The sealing film is for single-use only to avoid contamination between wells. Keep the substrate away from direct light to preserve its activity.
Follow the manual instructions carefully. The final results should be based on the microplate reader readings, not visual judgment.
All samples, washes, and waste materials should be treated as biohazardous. Do not mix reagents from different batches, as this may lead to inconsistent results.
In case of any conflict between the English and other language manuals, the English version takes precedence.
Proper handling and adherence to these guidelines are essential for accurate and reliable TAFI measurements. Always ensure a clean and organized workspace to minimize errors and maintain quality control throughout the procedure.
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