Thrombin-Activated Fibrinolysis Inhibitor (TAFI) Kit Instructions:
1. Before use, remove the kit from the refrigerator and let it reach room temperature for 15–30 minutes. If the enzyme label is opened, store the strip in a sealed bag to prevent contamination.
2. The concentrated washing solution may crystallize. When preparing the working solution, gently warm it in a water bath to dissolve the crystals. This will not affect the test results.
3. Use a dedicated pipette for each step and regularly check the accuracy to avoid errors. Try to complete sample loading within 5 minutes. For large sample volumes, a multichannel pipette is recommended.
4. Always prepare a standard curve during each test, ideally with duplicate wells. If the sample OD value exceeds that of the first standard well, dilute the sample before testing. Multiply the final result by the dilution factor (×n×5) when calculating.
5. The sealing film is for single-use only to prevent cross-contamination.
6. Keep the substrate away from direct light to maintain its stability.
7. Follow the manual strictly. The test results should be based on microplate reader readings.
8. Treat all samples, washes, and waste as potentially infectious materials.
9. Do not mix reagents from different batches.
10. In case of discrepancies between language versions, the English manual takes precedence.
Additional Notes:
It’s important to ensure proper storage conditions at all times. If the kit has been exposed to extreme temperatures or humidity, it may affect performance. Always check the expiration date before use. If any component appears damaged or discolored, do not use it. For best results, perform the assay in a clean and controlled environment. Also, make sure all reagents are thoroughly mixed before use. Proper training and adherence to safety protocols are essential for accurate and reliable outcomes.
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